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大肠埃希菌临床连续分离株常见耐药元件检测与分析

发布时间:2019-01-11 14:11
【摘要】:目的调查一组60株大肠埃希菌临床连续分离株常见耐药元件的携带情况和菌株间的亲缘性。方法收集2015年10月-12月医院住院患者分离的60株大肠埃希菌临床连续分离株,采用K-B法测定12种抗菌药物的敏感性,再用聚合酶链反应(PCR)及序列分析法分析15种β-内酰胺类、6种氨基糖苷类、3种磺胺类耐药相关基因以及4种可移动遗传元件遗传标记,并对检测结果作样本聚类分析。结果 60株大肠埃希菌对氨苄西林、庆大霉素、妥布霉素、环丙沙星、磺胺甲VA唑/甲氧苄啶耐药率50%,对其它7种抗菌药物的耐药率15%,对碳青霉烯类均敏感;60株菌共检出常见耐药元件基因15种,其中43株检出β-内酰胺类耐药基因,检出率为71.7%,33株检出氨基糖苷类耐药基因,检出率为55.0%,40株检出磺胺类耐药基因,检出率为66.7%,42株检出可移动遗传元件遗传标记基因,检出率为70.0%;共检出6种β-内酰胺类耐药基因,4种氨基糖苷类耐药基因,3种磺胺类耐药基因,2种可移动遗传元件遗传标记基因;样本聚类分析提示本组菌疑似存在8个克隆株,同一克隆内菌株携带着相同耐药元件,存在医院Qg感染。结论 blaTEM、aac(3)-Ⅱ、sul1、dfrA17、intⅠ1是导致本组菌株对β-内酰胺类、氨基糖苷类和磺胺类药物耐药的重要原因,耐药表型与基因型相符率高。
[Abstract]:Objective to investigate the common drug resistant elements and the relationship between 60 clinical isolates of Escherichia coli. Methods A total of 60 consecutive clinical isolates of Escherichia coli from October to December 2015 were collected and the sensitivity of 12 antimicrobial agents was determined by K-B method. Polymerase chain reaction (PCR) and sequence analysis were used to analyze 15 尾 -lactams, 6 aminoglycosides, 3 sulfonamides resistance related genes and 4 transportable genetic elements. Results 60 strains of Escherichia coli were resistant to ampicillin, gentamycin, tobramycin, ciprofloxacin, sulfamethoxazole / trimethoprim. Fifteen common resistant element genes were detected in 60 strains, among which 43 strains were detected 尾 -lactam resistance genes. The detection rate was 71.7% and 33 strains detected aminoglycoside resistance genes. The detection rate was 55.0%. 40 strains of sulfanilamide resistant genes were detected, and the detectable rate was 66.7% and 42 strains, the detectable rate was 70.010%. A total of 6 尾 -lactam resistant genes, 4 aminoglycoside resistant genes, 3 sulfonamides resistance genes and 2 transportable genetic element genetic marker genes were identified. Cluster analysis showed that 8 clones were suspected to exist in this group, and the strains in the same clone were carrying the same drug resistant elements, and there was nosocomial Qg infection. Conclusion blaTEM,aac (3)-鈪,

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