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Chemerin与炎症、动脉粥样硬化的相关性研究

发布时间:2019-01-09 14:13  文章来源:笔耕文化传播
【摘要】:目的:1、比较三组Wistar大鼠血浆Chemerin、HS-CRP、IL-6、TNF-p水平变化;观察三组Wistar大鼠主动脉Chemerin基因和Chemerin R基因表达情况。2、比较正常对照组和冠心病组之间血浆Chemerin水平,分析血浆Chemerin水平与临床一般、生化资料的相关性。方法:1、将健康雄性Wistar大鼠82只,分为对照组(D)、辛伐他汀治疗组(X)及高脂组(G)。喂养14周处死,行心脏采血、主动脉弓HE染色及分离胸主动脉和腹主动脉。2、ELISA法检测三组大鼠血浆Chemerin、HS-CRP、IL-6、TNF-β水平变化,采用半定量RT-PCR技术检测三组大鼠主动脉Chemerin-Chemerin R mRNA水平。3、用双抗体夹心法检测36例正常人和75例冠心病患者血浆Chemerin水平,同时测定受试者的体重指数(BMI)、腰臀比(WHR)、血压,检测血糖、血脂、超敏C反应蛋白(HS-CRP)等指标。结果:1、高脂组大鼠动脉粥样硬化(AS)形成良好。2、三组大鼠间血浆Chemerin、HS-CRP、IL-6、TNF-β水平差异具有统计学意义,均呈高脂组辛伐他汀治疗组对照组,P均0.01;高脂组大鼠血浆Chemerin与HS-CRP、IL-6、TNF-β呈正相关(r=0.804、0.614、0.821,P0.01),辛伐他汀治疗组与高脂组相似。3、三组大鼠间主动脉Chemerin-Chemerin R mRNA表达水平差异具有统计学意义,呈高脂组辛伐他汀治疗组对照组,P均0.01;高脂组、辛伐他汀治疗组Chemerin-Chemerin R mRNA表达水平与HS-CRP、IL-6、TNF-β相关性和相应组血浆Chemerin与HS-CRP、IL-6、TNF-β水平相关性相似。4、冠心病组与正常对照组血浆Chemerin水平差异具有统计学意义(P0.01),且与冠脉病变支数呈正相关(r=0.423,P0.05);在正常对照组,女性较男性有较高血浆Chemerin水平(P0.05);冠心病患者血浆Chemerin水平与HS-CRP、LDL呈正相关,r=0.512、0.246,P0.05;在校正年龄、性别、BMI、WHR、 BP后,血浆Chemerin与HS-CRP仍呈正相关(r=0.264,P0.05)。以Chernerin为因变量,作多元Logistic回归分析,有SBP、WHR、HS-CRP进入方程(P0.05)。结论:1、大鼠血浆Chemerin水平、主动脉Chemerin-Chemerin R基因mRNA表达水平均与炎症有明显相关性,而AS是一种慢性炎症过程,Chemerin与AS的形成可能具有某种内在联系。2、冠心病组Chemerin明显高于正常对照组,且与冠状动脉病变指数呈正相关,冠心病主要的病理是AS,提示这种新的脂肪因子可能在冠心病及其相关心血管疾病的病理生理机制中发挥作用。
[Abstract]:Objective: 1. To compare the changes of plasma Chemerin,HS-CRP,IL-6,TNF-p levels in three groups of Wistar rats. The expression of Chemerin gene and Chemerin R gene in aorta of three groups of Wistar rats were observed. 2. The plasma Chemerin level was compared between normal control group and coronary heart disease group, and the correlation between plasma Chemerin level and clinical and biochemical data was analyzed. Methods: 1. 82 healthy male Wistar rats were divided into control group, (D), simvastatin treatment group, (X) group and hyperlipidemic group (G). After 14 weeks of feeding, the rats were sacrificed, the blood was collected from the heart, the aortic arch was stained with HE, the thoracic aorta and abdominal aorta were separated, and the plasma Chemerin,HS-CRP,IL-6,TNF- 尾 levels in the three groups were detected by Elisa. The levels of Chemerin-Chemerin R mRNA in aorta of three groups of rats were measured by semi-quantitative RT-PCR technique. The plasma Chemerin levels of 36 normal subjects and 75 patients with coronary heart disease were detected by double antibody sandwich method. The body mass index (BMI),) was also measured. Blood pressure, blood glucose, blood lipid and high sensitive C reactive protein (HS-CRP) were measured in waist hip ratio (WHR),). Results: 1. The formation of atherosclerosis (AS) was good in the high fat group. 2There was significant difference in plasma Chemerin,HS-CRP,IL-6,TNF- 尾 level among the three groups. P both 0.01; There was a positive correlation between plasma Chemerin and HS-CRP,IL-6,TNF- 尾 in hyperlipidemia group (r = 0.804 / 0. 6140. 821 / P0.01), while simvastatin treatment was similar to that in hyperlipidemia group. The expression of Chemerin-Chemerin R mRNA in aorta of the three groups was significantly different (P < 0.01). The expression of Chemerin-Chemerin R mRNA in hyperlipidemia group and simvastatin group was similar to that of HS-CRP,IL-6,TNF- 尾 and plasma Chemerin and HS-CRP,IL-6,TNF- 尾 levels. There was significant difference in plasma Chemerin level between coronary heart disease group and normal control group (P0.01), and there was a positive correlation with the number of coronary artery disease branches (r = 0.423 P 0.05). In the normal control group, the plasma Chemerin level of female was higher than that of male (P0.05), the plasma Chemerin level of coronary heart disease patients was positively correlated with HS-CRP,LDL (r = 0.512, 0.246, P 0.05). After adjusting for age, sex and BMI,WHR, BP, plasma Chemerin was still positively correlated with HS-CRP (r = 0.264, P 0.05). With Chernerin as dependent variable, multiple Logistic regression analysis was made. SBP,WHR,HS-CRP entered the equation (P0.05). Conclusion: 1. The levels of Chemerin in plasma and mRNA expression of Chemerin-Chemerin R gene in aorta are significantly correlated with inflammation, while AS is a chronic inflammatory process, and the formation of Chemerin and AS may have some internal relationship. 2. The Chemerin of coronary heart disease group was significantly higher than that of normal control group, and had positive correlation with coronary artery disease index. The main pathology of coronary heart disease was AS,. These results suggest that this new fat factor may play a role in the pathophysiological mechanism of coronary heart disease and its related cardiovascular diseases.
【学位授予单位】:泸州医学院
【学位级别】:硕士
【学位授予年份】:2012
【分类号】:R363

【共引文献】

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