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衰老模型小鼠胰腺结构与功能的变化

发布时间:2019-01-11 08:19
【摘要】:目的探讨D-半乳糖(D-gal)致衰小鼠胰腺结构与功能变化。方法 2月龄雄性C57BL/6J小鼠随机分为两组,每组10只。衰老组:小鼠皮下注射D-gal(120mg/kg),每天1次,共42 d;对照组:小鼠皮下注射等时与等量生理盐水。衰老模型建立完成第2天,采外周血测定空腹血糖(FBG)与空腹胰岛素(FINS)水平;称小鼠体重(g)与胰腺湿重(mg)计算胰腺脏器指数;石蜡切片,HE染色观察胰腺光学显微镜下形态;制备胰腺冷冻切片,检测衰老相关β-半乳糖苷酶(SA-β-Gal)染色阳性胰腺细胞的相对吸光度(RA);免疫组织化学法观察胰腺组织晚期糖基化终产物(AGEs)的RA;制备胰腺组织匀浆检测超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)和丙二醛(MDA)的含量。结果衰老组小鼠FBG显著升高,FINS水平降低;胰腺湿重和胰腺脏器指数明显升高;胰腺光学显微镜下结构未见显著改变,但胰岛内单个有核细胞所占面积增加;胰腺SA-β-Gal染色阳性细胞数显著增加;AGEs阳性表达区域RA值明显升高;SOD与T-AOC含量显著降低,MDA含量显著升高。结论 D-gal复制衰老小鼠可致其胰腺损伤,其机制与D-gal致胰腺组织细胞的氧化应激损伤有密切关系。
[Abstract]:Objective to investigate the changes of pancreatic structure and function in aging mice induced by D-galactose (D-gal). Methods two-month old male C57BL/6J mice were randomly divided into two groups with 10 mice in each group. Aging group: mice were subcutaneously injected with D-gal (120mg/kg) once a day for 42 days; control group: mice were subcutaneously injected with the same amount of normal saline. On the second day after the establishment of the aging model, fasting blood glucose (FBG) and fasting insulin (FINS) were measured in peripheral blood, and the pancreatic organ index was calculated by weight (g) and wet weight (mg) of pancreas. Paraffin sections, HE staining to observe the morphology of pancreas under optical microscope; preparation of frozen sections of pancreas to detect relative absorbance of pancreatic cells with SA- 尾-Gal staining The content of total antioxidant activity (T-AOC) of superoxide dismutase (SOD),) and malondialdehyde (MDA) in pancreatic tissue homogenate was determined by immunohistochemical method. Results in aging group, FBG was significantly increased, FINS level was decreased, wet weight of pancreas and pancreatic organ index were significantly increased, the structure of pancreas was not changed significantly under optical microscope, but the area of single nucleated cells in pancreatic islets increased. The number of SA- 尾-Gal positive cells in pancreas was significantly increased, the RA value of AGEs positive region was significantly increased, the contents of SOD and T-AOC were significantly decreased, and MDA content was significantly increased. Conclusion D-gal replication can induce pancreatic injury in aging mice, and its mechanism is closely related to oxidative stress injury of pancreatic tissue cells induced by D-gal.
【作者单位】: 重庆医科大学干细胞与组织工程研究室组织学与胚胎学教研室;
【基金】:国家自然科学基金(30973818,81673748)
【分类号】:R-332;R339.38

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