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氯胺酮复合异丙酚麻醉对抑郁大鼠电休克后海马谷氨酸受体亚基1和亚基2的影响

发布时间:2019-01-12 11:06  文章来源:笔耕文化传播
【摘要】:目的评价小剂量氯胺酮复合异丙酚麻醉对抑郁大鼠电休克(electroconvulsive therapy,ECT)后海马谷氨酸受体亚基1(glutamate receptor subunit 1,Glu R1)及亚基2(glutamate receptor subunit 2,Glu R2)的影响。方法健康成年雄性SD大鼠32只,采用孤养与慢性轻度不可预见性应激(chronic unpredictable mild stress,CUMS)建立抑郁模型,建模成功后随机分为抑郁组(A组)、ECT组(B组)、ECT+异丙酚组(C组)和ECT+异丙酚+氯胺酮组(D组)4组,每组8只;另取8只正常大鼠作为对照组。对照组不做任何处理;A组腹腔注射生理盐水8 m L/kg后给予伪ECT处理;B、C、D组分别腹腔注射生理盐水8 m L/kg、异丙酚80 mg/kg、异丙酚80 mg/kg+氯胺酮10 mg/kg后行ECT处理,上述处理连续7 d。分别采用糖水偏好实验和Morris水迷宫实验评价大鼠抑郁状态和学习记忆功能,Western-blot及RT-PCR检测大鼠海马Glu R1和Glu R2及其m RNA表达。结果 ECT处理后,B、C、D组糖水偏好百分比变化均较A组和对照组明显,其中D组升高最为明显(均P0.05);B组逃避潜伏期延长且变化值与其他组均具有统计学差异,而D组逃避潜伏期缩短最为明显,其次为A组(均P0.05);B组空间探索时间缩短且变化值均大于其他组,D组空间探索时间延长最为明显(均P0.05)。ECT处理后,B、C、D组Glu R1表达较A组高,其中D组最高(均P0.05);Glu R1m RNA表达在组间无统计学差异B、C组Glu R2及其m RNA表达较其余组低,其中B组最低(均P0.05),A、D组和对照组间Glu R2及其m RNA差异无统计学意义(P0.05)。结论小剂量氯胺酮复合异丙酚麻醉在协同ECT抗抑郁效果的同时,能够更大程度地改善ECT后学习记忆功能,其机制可能与上调海马Glu R1和Glu R2表达相关。
[Abstract]:Objective to evaluate the effects of low-dose ketamine combined with propofol anesthesia on glutamate receptor subunit 1 (glutamate receptor subunit 1 Glu R1 and subunit 2 (glutamate receptor subunit 2Glu R2 in hippocampus of depressed rats after electroshock (electroconvulsive therapy,ECT). Methods Thirty-two healthy male SD rats were randomly divided into depression group (group A,), ECT group), and chronic mild unpredictable stress (chronic unpredictable mild stress,CUMS). ECT propofol group (group C) and ECT propofol ketamine group (group D) with 8 rats in each group. Another 8 normal rats were taken as control group. Group A was treated with pseudo-ECT after intraperitoneal injection of normal saline for 8 m L/kg. In group B, normal saline 8 mL / kg was injected intraperitoneally, propofol 80 mg/kg, propofol 80 mg/kg ketamine was treated with ECT for 10 mg/kg, and the above treatment lasted 7 days. The depression state and learning and memory function of rats were evaluated by sugar water preference test and Morris water maze test respectively. The expressions of Glu R1, Glu R2 and m RNA in hippocampus of rats were detected by Western-blot and RT-PCR. Results after ECT treatment, the percentage change of sugar water preference in group D was significantly higher than that in group A and control group, especially in group D (P0.05). The escape latency of group B was prolonged and the change value was significantly different from that of other groups, while the escape latency of group D was shortened most obviously, followed by group A (P0.05). The time of space exploration in group B was shorter than that in other groups, and the time of space exploration in group D was the most obvious (P0.05). After treatment with). ECT, the expression of Glu R1 in group D was higher than that in group A, among which the expression of Glu R1 in group D was the highest (P0.05). There was no significant difference in the expression of Glu R1m RNA between the two groups. The expression of Glu R2 and m RNA in group B was lower than that in group B (P0.05), but there was no significant difference in Glu R2 and m RNA between group A and control (P0.05). Conclusion low dose ketamine combined with propofol anesthesia can significantly improve the learning and memory function after ECT in combination with the antidepressant effect of ECT. The mechanism may be related to the up-regulation of the expression of Glu R1 and Glu R2 in hippocampus.
【作者单位】: 重庆医科大学附属第一医院麻醉科;
【基金】:国家自然科学基金面上项目(编号:81271501) 国家临床重点专科建设项目经费资助(编号:财社(2011)170号) 重庆市科委应用开发项目经费资助(编号:cstc2014yykf A110028)
【分类号】:R614

【共引文献】

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3 张喜梅;陈景旭;;非单胺靶标抗抑郁药的研究进展[J];国际精神病学杂志;2014年03期

4 王辉;张志s,

本文编号:2407704


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