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一种新的冷冻夹具设计及其在大鼠跟腱力学测试中的应用

发布时间:2019-01-11 08:31  文章来源:笔耕文化传播
【摘要】:跟腱疾病通常是由于过度劳损造成的,常发生于体育运动、繁重体力劳动等场合,呈逐年递增趋势。肌腱损伤常常不能自愈,治疗这一运动疾病需要生物工程学家的参与。本研究设计了一种新的、精确易用的冷冻夹具,解决了以往肌腱损伤修复基础研究中力学测试困难的困境。该冷冻夹具通过液氮将预冷的肌肉在力学测试过程中保持在冷冻状态,从而解决了拉伸测试过程中肌肉/肌腱的相对滑移和应力集中问题。该夹具还设计了一个生理盐水浴环境,从而使整个测试过程很好的模拟了生理环境,保证了体外测试数据能真实体现体内生理状况。 本研究以大鼠跟腱为对象,利用设计的冷冻夹具进行拉伸测试。共20个待测试样,随机分为4组,分别测试不同预冷方法、样品拉伸环境、夹具移动速度下跟腱的力学特性。具体分组和测试方法如下:A组,将样品放置-20℃冷冻1周,生理盐水浴环境拉伸,夹具移动速度为10mm/min;B组,将样品放置液氮速冻1分钟,生理盐水浴环境拉伸,夹具移动速度10mm/min;C组,将样品放置液氮速冻1分钟,室温(25℃)空气环境拉伸,夹具移动速度10mm/min;D组,将样品放置液氮速冻1分钟,室温(25℃)空气环境拉伸,夹具移动速度500mm/min。记录力-位移曲线,本研究获取了4组样品的最大断裂力、断裂点最大位移、杨氏模量,结果分别如下:A组,119.2±25N,1.868±0.174mm,264.64±108.78MPa;B组,90.5±13N,3.6838±0.8825mm,66.926±24.743MPa;C组,70±20N,2.966±1.279mm,86.822±27.915MPa;D组,53±10N,2.130±0.719mm,38.078±9.765MPa。结果经过统计分析表明:-20℃冷冻1周和液氮速冻1分钟的样品处理方式最大断裂力无显著差异,生理盐水浴的拉伸环境最大断裂力显著大于室温(25℃)空气环境,夹具移动速度在低速(10mm/min)和高速(500mm/min)时最大断裂力无显著差异,但低速时断裂点最大位移更均一。
[Abstract]:Achilles tendon disease is usually caused by overstrain, often occurs in sports, heavy physical labor and other occasions, showing an increasing trend year by year. Tendon injuries often fail to heal, and the treatment of the disease requires the involvement of bioengineers. In this study, a new, accurate and easy to use freezing fixture was designed to solve the difficulty of mechanical testing in previous basic researches of tendon repair. The freezing fixture keeps the precooled muscles frozen in the mechanical testing process by liquid nitrogen, thus solving the problems of relative slip and stress concentration of the muscle / tendon during the tensile testing. The fixture also designed a physiological saline bath environment, so that the whole testing process is very good simulation of the physiological environment, to ensure that in vitro test data can truly reflect the physiological situation in vivo. In this study, the Achilles tendon of rats was used for tensile test with the designed freezing fixture. A total of 20 samples were randomly divided into 4 groups. The mechanical properties of Achilles tendon under different precooling methods, tensile environment and moving speed of clamp were tested respectively. The specific grouping and testing methods were as follows: group A, the samples were frozen at -20 鈩,

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